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Studies that have measured GDH activity and exogenous glutamate use in primary neuronal cultures from the rat ventral mesencephalon have shown that GDH activity is approximately four times higher in mature (12-day-old) cultures than in immature (4-day-old) cultures and have identified a significant correlation between exogenous glutamate use (added to the medium at 1.2 mM) and GDH activity in cultured cells (Plaitakis and Shashidharan, 2000)

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