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Samples were centrifuged for 10 min at 200 g at 4°C, the supernatant was removed, and the cell pellet was resuspended in 2% BSA in PBS and the suspension was centrifuged for 10 min at 200 g at 4°C

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Let’s look at their relationship with the ovary (which under normal conditions produces estradiol, progesterone, and testosterone in women) (5)