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Long-term treatment with metformin in type 2 diabetes and methylmalonic acid: post hoc analysis of a randomized controlled 4.3 year trial

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Materials & methods Cells Native healthy normal fibroblasts (NFs) and primary CAFs from different donors and tissues - pancreatic (CAF1 and NF1), breast (CAF2 and NF2), lung (CAF3 and NF3) and colorectal (CAF4 and NF4) - were selected to investigate a broad range of fibroblasts, whereas CAFs and NFs were not isolated from the same donor (all CAFs and NF1 used in this study were obtained from Vitro Biopharma (Golden, CO, US) with the following catalog numbers: CAF1 (cat# CAF08), CAF2 (cat# CAF06), CAF3 (cat# CAF07-AD), CAF4 (cat# CAF05), and NF1 (cat# SC00A5))