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For intracellular staining, the lung cells were fixed and permeabilized using BD Cytofix/Cytoperm buffer first, α‐SMA and h‐caldesmon antibodies were used to detected myofibroblasts and smooth muscle cells, the mean fluorescence intensity (MFI) of α‐SMA and h‐caldesmon was detected by a BD Aria Ⅲ flow cytometer (BD Bioscience)