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To prevent this damage, organisms from all domains of life have antioxidants that function to detoxify and regulate ROS in reduction reactions 6

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1, to synthesize 1 mole of glutamate, 0.5 mole of aerial nitrogen is fixed by nitrogenase with the consumption of 8 moles of ATP and fixed ammonia is assimilated into glutamate by the GS-GOGAT system with the consumption of 1 mole of ATP