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In brief, the wells of a 96-well Nunc maxisorp microtitre plate were coated with 250 ng desmosine-egg-albumin complex (Elastin Products Company (EPC), Missouri, USA) in 100 µl bicarbonate buffer, pH 9.6 (Sigma-Aldrich), overnight at 4 °C

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Additional proteins associated with lipid droplets (LD) in adipocytes participate in the ABHD5-mediated regulation of ATGL