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The residual hematocrit was mixed immediately with 1 mL chilled, isotonic MBB (purchased from Calbiochem) buffer solution (pH 7.5) containing the following: 5 mmol MBB/L, 17.5 mmol Na 2 EDTA/L, 50 mmol potassium phosphate/L, 50 mmol serine/L, and 50 mmol boric acid/L

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N -acetylglutamate for instance is synthesized from the amino acid glutamate, and this compound is acting as an allosteric cofactor for carbamylphosphate synthetase I, an enzyme involved in the liver urea cycle [3]