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The gRNAs were expressed using an U6gRNA-Cas9-2A-RFP (Sigma-Aldrich), or pSpCas9 BB-2A-Puro (PX459) v2.0 vector after transient transfection using Lipofectamine LTX following the manufacturer’s protocol

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Preclinical studies indicate a potential use of HIF therapeutics to treat inflammatory diseases, such as inflammatory bowel disease

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