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Similarly, another study employing LLC-PK1 cells transfected to express PC1 and PC2 employed a pulse-chase imaging approach to examine the delivery of PC1 to the cilium and to the plasma membrane reported that treatments that perturb trafficking of newly synthesized proteins through the Golgi complex, including brefeldin A (187, 188) or by incubation at 20° C (189, 190), prevent the delivery of PC1 to the plasma membrane but do not interfere with its delivery to the cilium (124)

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J Dtsch Dermatol Ges (2022) 20(2):218–21